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2 at 25°C Suspend 23 gram powdered medium in 1 litre distilled water
Ferric chloride
69 Store dry at room temperature
Chemical formula C5H10N2O3 Solubility (20° C) 26 g/l Mol
The addition of a carbon source is required
Vegetal LoFlo Medium Size:1000g 2 at 25°C Suspend 23LoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to
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