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The overnight culture was then diluted 1:100 in MuellerHinton broth and 200 l applied to sterile Whatman cyclopore circular membrane 0.2 m (Whatman plc, Maidstone, UK) placed on Mueller-Hinton agar plates (Sigma-Aldrich Company Ltd, Kent, UK) at 37 C for 24 h to form colony biofilms

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