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SDS-polyacrylamide gel electrophoresis (SDS-PAGE) For SDS-PAGE, each sample was first mixed with an equal volume of a protein denaturing solution (100 mM TrisHCl (pH 6.8), 20% glycerol, 2% SDS, 0.86 M 2-mercuptoethanol, and 0.2 mM phenylmethylsulfonyl fluoride)

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